Biotechnology and its Application 5 Sample Paper with Answer Sheet

Agrobacterium tumefacien, a natural pathogen of Several dicot plants is able to deliver a piece of DNA known as “t – DNA” to transform normal plant cell into a tumor & direct gene transfer transform tumor cells to produce chemicals required by pathogen . The tumor inducing (Ti) plasmid of Agrobacterium tumefacien has now been modified into a cloning vector which is no more pathogenic to plant but is still able to use the mechanism to deliver genes of our interest into a variety of plants